184 Activated Macrophages in Antibody

نویسندگان

  • LINDA BRUKNER
  • GILLA KAPLAN
  • JAY UNKELESS
  • ZANVIL COHN
چکیده

Mononuclear leukocytes are able to destroy tumor cells under a variety of circumstances. In few such instances is the biochemical basis of cytotoxicity understood. Recently, we demonstra ted that activated macrophages, s t imulated by phorbol myristate acetate (PMA), 1 secreted substantial amounts o f hydrogen peroxide (1). The release o f this oxidant from macrophages correlated with the extracellular lysis o f tumor cells (2). In fact, under the conditions tested, hydrogen peroxide was both necessary and sufficient for cytolysis by activated macrophages (3). The secretion by phagocytic leukocytes of toxic species o f reduced oxygen can be triggered not only pharmacologically, but also immunologically, such as by contact with ant ibodycoated particles or aggregated immunoglobul in (4-9). Therefore, we a t tempted to learn whether activated macrophages would lyse tumor cells when the tumor cells were coated with homologous antibody, and whether reactive metabolites of oxygen would play a role in this form of cytolysis. Tests of these hypotheses are reported below and in the accompanying paper (10).

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تاریخ انتشار 2003